The effect of Eurycoma Longifolia on the regulation
of reproductive hormones in young males

Kai Quin Chan, Claire Stewart, Neil Chester, Sareena H Hamzah, Ashril Yusof


Eurycoma longifolia supplementation increases testosterone levels in humans via activation of the hypothalamic-pituitary-gonadal axis and/or the hypothalamic-pituitary-adrenal axis mainly in older adults and nonhealthy populations. This study aimed to assess the impact of Eurycoma longifolia on the hypothalamic-pituitary-gonadal and hypothalamic-pituitary-adrenal axes in healthy young males since this might promote functional testosterone prowess. Thirty-two males (24.4 ± 4.7 years; 1.74 ± 0.07 m; 73.7 ± 8.4 kg) in a placebo-controlled, double-blind, matched-paired study received 600 mg/day Eurycoma longifolia or placebo for two weeks. Blood analysis using repeated measures analysis of variance showed significant interaction and time effects for testosterone (F1,30 = 9.04, p = .005), free testosterone (F1,30 = 7.13, p = .012) and estradiol (F1,30 = 8.07, p = .008) levels in favour of the treatment group, while luteinising hormone, follicle-stimulating hormone and sexual hormone-binding globulin did not. The lack of changes in luteinising hormone and follicle-stimulating hormone levels suggests that a lesser role played by Eurycoma longifolia in activating the hypothalamic-pituitary-gonadal axis in the young adults. The raised testosterone level may be due to a greater rate of hormone production via the hypothalamic-pituitary-adrenal axis. The supplementation of Eurycoma longifolia for two weeks demonstrates steroidogenic effects on young men were dose-related. Consequently, the raised testosterone following Eurycoma longifolia supplementations could benefit muscle and strength gain in young adults.


Aguilera, R., Chapman, T. E., Starcevic, B., Hatton, C. K., & Catlin, D. H. (2001), Performance characteristics of a carbon isotope ratio method for detecting doping with testosterone based on urine diols: Controls and athletes with elevated testosterone/epitestosterone ratios, Clinical Chemistry, Vol 47(2), Pages 292-300

Azarneoshan, F., KhatamsazSaz, S., & Sadeghi, H. (2009), The effects of hydro alcoholic extract of doremaaucheri on blood concentration of gonadotropin and androgen hormones in adult rats, Armaghane Danesh, Vol 14(3), Pages 65-70.

Bashir, M. A., Salleh, F. M., & Wagiran, A. (2017), Chemical composition of Eurycoma longifolia (Tongkat Ali) and the quality control of its herbal medicinal products, Journal of Applied Sciences, Vol 17(7), Pages 324-338.

Bhat, R., & Karim, A. A. (2010), Tongkat Ali (Eurycoma longifolia Jack): A review on its ethnobotany and pharmacological importance, Fitoterapia, Vol 81(7), Pages 669-679.

Binder, S. (2012), Loss of reactivity in intravitreal anti-VEGF therapy: Tachyphylaxis or tolerance? British Journal of Ophthalmology, Vol 96(1).

Chen, C. K., Ooi, F. K., Abu Kasim, N. A., & Asari, M. A. (2019), Effects of Eurycoma longifolia Jack supplementation combined with resistance training on isokinetic muscular strength and power, anaerobic power, and urinary testosterone: Epitestosterone ratio in young males, International Journal of Preventive Medicine, Vol 10(118), Pages 1-8.

Chen, C. K., Wan Mohamad, W. M. Z., Ooi, F. K., Ismail, S. B., Abdullah, M. R., & George, A. (2014), Supplementation of Eurycoma longifolia Jack extract for 6 weeks does not affect urinary testosterone: Epitestosterone ratio, liver and renal functions in male recreational athletes, International Journal of Preventive Medicine, Vol 5(6), Pages 728-733.

Chen, S., Wang, J., Yu, G., Liu, W., & Pearce, D. (1997), Androgen and glucocorticoid receptor heterodimer formation. A possible mechanism for mutual inhibition of transcriptional activity, Journal of Biological Chemistry, Vol 272, Pages 14087-14092.

Darise, M., Kohda, H., Mizutani, K., & Tanako, O. (1982), Eurycomanone and eurycomanol quassinoids from the root of Eurycoma longifolia, Phytochemistry, Vol 21, Pages 2091-2093.

de Ronde, W., & de Jong, F. H. (2011), Aromatase inhibitors in men: Effects and therapeutic options, Reproductive Biology and Endocrinology, Vol 9(93), Pages 1-7.

Tongkat Ali, Indonesian Root Grade A 1:200 Extract

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